{"id":3477,"date":"2020-10-20T12:43:00","date_gmt":"2020-10-20T10:43:00","guid":{"rendered":"https:\/\/wppruebas.i3a.es\/?p=3477"},"modified":"2023-09-12T14:15:33","modified_gmt":"2023-09-12T12:15:33","slug":"automatic-quantification-of-cardiomyocyte-dimensions-and-connexin-43-lateralization-in-fluorescence-images","status":"publish","type":"post","link":"https:\/\/bsicos.i3a.es\/es\/automatic-quantification-of-cardiomyocyte-dimensions-and-connexin-43-lateralization-in-fluorescence-images\/","title":{"rendered":"Automatic quantification of cardiomyocyte dimensions and connexin 43 lateralization in fluorescence images"},"content":{"rendered":"
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    Noticias<\/a><\/h3>\n

    Automatic quantification of cardiomyocyte dimensions and connexin 43 lateralization in fluorescence images\n<\/strong><\/h1>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/li>\n\t\t<\/ul>\t\t\t\t
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      Oct 20, 2020<\/em><\/strong><\/p>\n

      Cardiomyocytes\u2019 geometry and connexin 43 (CX43) amount and distribution are structural features that play a pivotal role in\u00a0electrical conduction.<\/strong><\/b>\u00a0Their quantitative\u00a0assessment is of high interest in the study of arrhythmias, but it is usually hampered by the lack of automatic tools. In this work, we propose\u00a0a software algorithm<\/strong><\/b>\u00a0(Myocyte Automatic Retrieval and Tissue Analyzer, MARTA) to\u00a0automatically detect myocytes<\/strong><\/b>\u00a0from fluorescent\u00a0microscopy images of cardiac\u00a0tissue,<\/strong><\/b>\u00a0measure their morphological features and evaluate the expression of\u00a0CX43 and its degree of lateralization.<\/p>\n

      The proposed software is\u00a0based on the generation of cell masks<\/strong><\/b>, contouring of individual\u00a0cells, enclosing of cells in minimum area rectangles and splitting of these\u00a0rectangles into end-to-end and middle compartments to estimate CX43\u00a0lateral-to-total ratio. Application to\u00a0human ventricular tissue<\/strong><\/b>\u00a0images shows\u00a0that mean differences between automatic and manual methods in terms of
      \ncardiomyocyte length and width are below 4 \u00b5m.<\/p>\n

      The\u00a0percentage of lateral CX43 also agrees between automatic and manual evaluation,\u00a0with the interquartile range approximately covering from 3% to 30% in both cases.\u00a0MARTA<\/strong><\/b>\u00a0is not limited by fiber orientation and has an optimized speed by using contour filtering, which makes it run hundreds of times faster than a trained\u00a0expert. Developed for CX43\u00a0studies in the left ventricle,<\/strong><\/b>\u00a0MARTA is a flexible tool applicable to morphometric and lateralization studies of other markers in\u00a0any heart chamber or even skeletal muscle. This\u00a0open-access<\/strong><\/b>\u00a0software is\u00a0available online.<\/p>\n

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      A.\u00a0Oliver-Gelabert, L. Garc\u00eda-Mend\u00edvil, J.M. Vallejo-Gil, P. C. Fresneda-Rold\u00e1n, K. Andelov\u00e1, J. Fa\u00f1an\u00e1s-Mastral, M. V\u00e1zquez-Sancho, M. Matamala-Adell, F.
      \nSorribas-Berj\u00f3n, C. Ballester-Cuenca, N. Tribulova, L. Ordov\u00e1s, E.R. Diez, E.\u00a0Pueyo (2020). Automatic quantification of cardiomyocyte dimensions and connexin
      \n43 lateralization in fluorescence images. Biomolecules. 2020, 10(9), 1334<\/p>\n<\/blockquote>\n

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      Full Article:\u00a0https:\/\/doi.org\/10.3390\/biom10091334<\/a><\/p>\n<\/div>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>\n\n

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