in-vitro-modelling
by

Research line

Experimental characterization and in-vitro modeling of human cardiac aging for:

  1. Temporal and spatial variability characterization of the electrophysiology of the human ventricle based on age
  2. Studying of structural, molecular and autonomic changes in human ventricles induced by aging
  3. Creating, characterizing and validating an aging in-vitro model based on human iPSC
  4. Searching for anti-aging targets at molecular level which explain electrophysiological changes linked to age
  5. Analyzing the effects of aging on congenital cardiomiopathies


Video: BSICoS uses human iPSC-derived myocytes for cardiac research

in-vitro-modelling

Investigator in charge

Laura Ordovás Vidal

Laura Ordovás Vidal

Technologies and targeting sectors

  • Technology 1

Cardiac biomarkers as non-invasive tools to predict heart dysfunction in relation to age, heart damage or disease.

Targeting sector: Pharmaceutical and diagnostics companies.

  • Technology 2

Discovery of cardiac anti-aging interventions for the development of novel therapies.

Targeting sector: Pharmaceutical companies

Singular equipment and techniques

Human iPSC cell culture lab and genome engineering techniques

Optical Mapping (MiCAM O5-Ultima CMOS camera, SciMedia, Costa Mesa, CA) for electrophysiological recording

Vibratome (VT12000 s, LEICA) for the preparation of myocardial tissue slices

Mechanical electrostimulation system (C-Strecht and C-Pace M, Ion Optix) for organotypic tissue and cell culture

Projects

Translational cardioselective RNAi therapies and predictive heart-on-chip to tackle cardiac clinical challenges (ADVANCE) – PID2022-139859OB-I00

Translational cardioselective RNAi therapies and predictive heart-on-chip to tackle cardiac clinical challenges (ADVANCE) – PID2022-139859OB-I00

Customised DNA-based nanocarriers to boost heart healing (DNABEATS) – PCI 2023

Customised DNA-based nanocarriers to boost heart healing (DNABEATS) – PCI 2023

Computational biomechanics and bioengineering 3D printing to develop a personalized regenerative biological ventricular assist devide to provide lasting functional support to damaged hearts – BRAV3

Computational biomechanics and bioengineering 3D printing to develop a personalized regenerative biological ventricular assist devide to provide lasting functional support to damaged hearts – BRAV3

Finished Projects

Equipment for the chronic electro-mechanical stimulation of cell and tissue cultures that allows their maintenance under biomimetic conditions. Force transducer and controller to program duration and amplitude of mechanical stretch

Human cardiac ageing modeling and identification of age related electrophysiological markers

Multi-Scale Physiology-Driven Computational Tools To Assist In The Assessment And Management Of Cardiac Dysfunctions (MScPhCarDys)

Equipment for Dual Optical Mapping of Transmembrane Potential and Calcium Transient

ERC Starting Grant. Is your heart aging well? A systems biology approach to characterize cardiac aging from the cell to the body surface (MODELAGE)

Towards improved management of cardiovascular diseases by integrative in silico-in vitro-in vivo research into hearts structure, function and autonomic regulation – PID2019-105674RB-I00

Straight to the heart: development of cardio-selective microRNA nanotherapies (DIANA) – LMP128_21

Confocal microscope with high performance Light Sheet based system and toxicity assessment platform